Research Technologies Life Sciences (RTLS) is the only area within RT that is dedicated to Life Sciences research.

Its primary mission is to empower those researchers with cutting edge, enabling information technologies in both basic and biomedical life sciences within IU, the state of Indiana, and beyond. RTLS consists of three subunits: the Advanced Information Technology Core, which acts as the gateway for IU School of Medicine and translational researchers to all RT systems and services; Biomedical Applications, which provides custom applications and services for pre-clinical and clinical research and; Bioinformatics and Computational Biology, which supports basic biology researchers on the Bloomington campus.

It has a secondary mission to create novel cyberinfrastructures that advance scholarship at IU, in Indiana, and beyond. These include novel analytical, data management, and virtual collaboration environments.